S. Jeznienė “Evaluation of probiotic properties of Lactobacillus reuteri, Lactobacillus plantarum and Lactobacillus helveticus isolated from sourdough, their encapsulation in biopolymeric systems and application in cosmetics” doctoral dissertation defense

Thesis defense

Author, Institution: Sigita Jeznienė, Kaunas University of Technology

Science area, field of science: Technological Sciences, Chemical Engineering, T005

Scientific Supervisors: Prof. Dr. Aušra Šipailienė (Kaunas University of Technology, Technological Sciences, Chemical Engineering, T005) 2016 – 2023 m. m.

Prof. Dr. Jolanta Liesienė (Kaunas University of Technology, Technological Sciences, Chemical Engineering, T005) 2015 – 2016 m. m.

Dissertation Defense Board of Chemical Engineering Science Field:
Assoc. Prof. Dr. Joana Bendoraitienė (Kaunas University of Technology, Technological Sciences, Chemical Engineering, T005) – chairperson
Assoc. Prof. Dr. Loreta Bašinskienė (Kaunas University of Technology, Technological Sciences, Chemical Engineering, T005)
Assoc. Prof. Dr. Viktorija Eisinaitė (Kaunas University of Technology, Technological Sciences, Chemical Engineering, T005)
Dr. Egidija Rainosalo (Centria University of Applied Science, Finland, Technological Sciences, Chemical Engineering, T005)
Prof. Dr. Lina Raudonė (Lithuanian University of Health Sciences, Medical and Health Sciences, Pharmacy, M003)

Dissertation defense meeting will be at Rectorate Hall of Kaunas University of Technology (K. Donelaičio 73 – 402, Kaunas)

 

The doctoral dissertation and summary of doctoral dissertation are available at the library of Kaunas University of Technology (Gedimino 50, Kaunas)

 

Annotation: One of the alternative therapies gaining momentum in healthcare is probiotic therapy. The protection provided by these probiotic bacteria against pathogenic microorganisms is the basis of probiotic therapy. In the cosmetic industry, it is challenging to develop formulations that maintain the viability of probiotics from production to consumer and throughout the claimed shelf life of the product. One of the ways to maintain viable microorganisms in these systems is the encapsulation of probiotic bacteria. The aim of this thesis was to evaluate the probiotic properties of Lactobacillus reuteri, Lactobacillus plantarum and Lactobacillus helveticus isolated from spontaneous bread sourdough, to encapsulate cultures in different biopolymeric systems and to evaluate the potential for the practical application in cosmetics. The probiotic properties of the studied lactic acid bacteria, such as autoaggregation and coaggregation, antagonistic effect on pathogenic microorganisms, inhibition of pathogenic biofilms, ability to colonise the human skin, etc., were assessed. Bacteria were encapsulated in double emulsions and calcium alginate gel capsules. After determining the encapsulation efficiency, survival of bacteria during freeze-drying, physical and mechanical properties of the capsules and after evaluating the viability of bacteria during long-term storage and the properties of probiotic bacteria, one bacterial culture and one encapsulation system were selected. Thus, the encapsulated Lactobacillus reuteri bacteria in calcium alginate gel capsules was evaluated for the practical application in model probiotic cosmetic products.

June 19 d. 13:00

Rectorate Hall at Kaunas University of Technology (K. Donelaičio 73-402, Kaunas)

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